Agrobacterium rhizogenes-Mediated Transformation: Root Cultures as a Source of Alkaloids
نویسندگان
چکیده
منابع مشابه
Transformation of Catalpa ovata by Agrobacterium rhizogenes and phenylethanoid glycosides production in transformed root cultures.
Transformed root cultures of Catalpa ovata were established following shoots infection with four agropine strains of Agrobacterium rhizogenes. Frequency of root formation was dependent on the bacterial strain and the presence of acetosyringone in the incubation medium. It is the first report concerning the possibility of transforming Catalpa ovata by A. rhizogenes. Both transformed and untransf...
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BACKGROUND Plants within the Orobanchaceae are an agriculturally important group of parasites that attack economically important crops to obtain water and nutrients from their hosts. Despite their agricultural importance, molecular mechanisms of the parasitism are poorly understood. METHODOLOGY/PRINCIPAL FINDINGS We developed transient and stable transformation systems for Phtheirospermum jap...
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Using Agrobacterium rhizogenes due to create hairy roots is a useful method to product secondary metabolites in many medicinal plants. The transgenic hairy roots were induced from Peganum multisectum (Maxim) Bobrov a medicinally important species, by infecting leaf and stem explant with wild type Agrobacterium rhizogenes strain ATTCC 15834, which led to the induction of hairy roots from 19% of ...
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Linum spp. (Linaceae) has been shown to accumulate cytotoxic lignans such as podophyllotoxin and 6-methoxypodopyllotoxin. Therefore, plant cell and tissue cultures of Linum spp. may be possible alternative sources for the lignan production. In this study, we focused on the production of lignans by root cultures of Linum spp. Hairy root cultures of L. nodiflorum, L.album and L. austriacum and ro...
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In this work, Isatis tinctoria hairy root cultures (ITHRCs) were established as an alternative source for flavonoids (FL) production. I. tinctoria hairy root line V was found to be the most efficient line and was further confirmed by the PCR amplification of rolB, rolC and aux1 genes. Culture parameters of ITHRCs were optimized by Box-Behnken design (BBD), and eight bioactive FL constituents (r...
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ژورنال
عنوان ژورنال: Planta Medica
سال: 2002
ISSN: 0032-0943,1439-0221
DOI: 10.1055/s-2002-34924